Pre-Conference Workshop Day

WORKSHOP A

Designing Target-Specific gRNAs for the CRISPR Genome Editing System Including Base Editors and Prime Editors using CRISPRseek and GUIDEseq
9:00– 12:00

CRISPR technology is revolutionizing biological studies and holds tremendous promise for therapeutic applications. To facilitate the adoption and improvement of this technology, CRISPRseek has been developed for designing target specific high efficacy gRNAs, and GUIDEseq for identifying genome-wide off-target sites from GUIDE-seq and CIRCLE-seq experiments to assess the precision of engineered CRISPR-Cas9 nucleases.

Attend this workshop to learn about:

  • Designing target-specific high efficacy gRNAs for the CRISPR-Cas system from different species, different variants or different configuration using CRISPRseek
  • Designing gRNAs for base editors and the prime editor using CRISPRseek
  • Designing CRISPR screening libraries
  • Designing allele-specific gRNAs using CRISPRseek
  • Analyzing GUIDE-seq and CIRCLE-seq data using GUIDEseq

Workshop Leader

Lihua Julie Zhu

Lihua Julie Zhu
Professor & Head of Bioinformatics Core
University of Massachusetts

WORKSHOP B

Applying the Expanding CRISPR Toolbox to Improve Screening Techniques for Drug Discovery and Beyond
13:00 – 16:00

CRISPR has drastically improved screening techniques, but there are still challenges surrounding more specific mutations, creating multiple edits to screen through more mutations, and scaling up screening. With novel CRISPR technologies being
rapidly developed, it is key that screening takes advantage of these to keep progressing, in drug discovery and beyond.

Attend this workshop to learn about:

  • Creating knock-in CRISPR screens
  • Making multiple edits to screen through more mutations
  • Using pooled CRISPR screens
  • Using screening to search for selection signatures

Workshop Leaders

alejandro amador

Alejandro Amador
Head of High Throughput Biology, Functional Genomics
GlaxoSmithKline

Rui Wang

Rui Wang
Principal Research Scientist
Abbvie